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Assessment of Cell Viability ARPE-19 cell viability was evaluated by performing the 3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)2-(4-sulfophenyl)2 H-tetrazolium (MTS) assay as previously described [25]

Keywords: GHK peptide, fibroblasts, myofibroblasts, senescence, fibrosis, idiopathic pulmonary fibrosis The loss of cellular homeostasis in fibrosis is characterized by the accumulation of activated fibroblasts, known as myofibroblasts [1], which are responsible for excessive extracellular matrix deposition and tissue remodeling

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Cells were then analyzed with the MTT viability assay, the live/dead assay, or processed for protein or RNA isolation as described below

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