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Formation of HO causes chronic pain, reduces range of motion, and is often treated through surgical resection, which is associated with a high likelihood of relapse (6, 7)

The enzyme reaction was measured in a cuvette containing 820 l phosphate buffer (66 mmol/L, pH 7.0), 35 l enzyme solution, 105 l of 50 mmol/L glutathione (GSH), and 40 l of 30 mmol/L 1-chloro-2,4-dinitrobenzene (CDNB), and changes in absorption at 340 nm were recorded for 5 min

doi: 10.3390/nu14204334, 141

for more detailed discussions see [50], [51]

"DPP-4 Inhibitors for Type 2 Diabetes: Drug Safety Communication - May Cause Severe Joint Pain"