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doi: 10.1186/s12967-018-1644-y 100 SospedraMMartinR

Our results indicate that the mean age in the post-protocol year was significantly higher than that in the pre-protocol year (regardless of the serum level)

After grinding and mixing, the homogenate was placed on ice for 10 min for full lysis

Xue Yaqi WZ, Liang Peiyi

For the actual experiments, multi-well plates or flasks (Thermo Fisher Scientific) were pre-coated with 0.1 mg/mL PLO (37 C, overnight) and subsequently with 5 g/mL fibronectin (FN