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Imaging glutathione depletion in the rat brain using ascorbate-derived hyperpolarized MR and PET probes

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The above experiment was repeated in presence of 10 l of liver supernatant (360 g protein ml -1 ) that contained glutathione transferase and small amount of GSH

Pretreatment with the RIPK1 inhibitors necrostatin-1 or necrostatin-1s or the MLKL inhibitor necrosulfonamide prevented phosphorylation of RIPK1 after DC661 (1 M) treatment in melanoma cells (Figure 2G) but failed to rescue DC661-induced cytotoxicity in melanoma cells (Figure 2H) or colon cancer or pancreatic cancer cells (Supplemental Figure 2G)