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MTX-induced apoptosis was significantly attenuated with all treatments except for NTZ-100, where, as depicted in Figures 5AD, Bcl-2 level was augmented while Bax was reduced, and consequently, Bax/Bcl-2 ratio was also reduced as well as caspase-3 activity all when compared to MTX group

Protein damage tryptophan fluorescence (decrease in OD)

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In this assay, GPX reduces hydroperoxides, producing oxidized glutathione (GSSG), which is then recycled back to its reduced state by glutathione reductase (GR) and NADPH

Two dimension electrophoresis (2D-PAGE) Platelet fractions were lysed with 150 L of protein lysis buffer containing 7 M urea, 2 M thiourea, 4% CHAPS, 100 mM DTT, and 1% human protease inhibitor cocktail