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72 h after transfection, cells were harvested by trypsin digestion and re-suspended in 100 uL of 1 PBS

Intranasal administration is valued as a non-invasive means of both systemic and local administration with good bioavailability, rapid onset, and targeted effects on the central nervous system [13, 14]

Vitamin B12 injections work quickly and efficiently by bypassing the digestive system and delivering the nutrient directly into the bloodstream, making them an excellent choice for those with absorption issues

Similarly, the reduced content of GSH and decreased activity of antioxidant enzymes in the intestine (decreased GR, GPx and SOD activity) (Reference Chen, Feng and Jiang34) and gills (decreased GR, GPx, SOD and CAT activity) (Reference Chen, Feng and Jiang35) of riboflavin-deficient fish have been reported

5 children (15.1%) had intolerable side effects and were withdrawn