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3.2.1 PCLS as a research tool for oxidative stress and inflammation The robust oxidative stress and sterile inflammation occurring upon reperfusion is a key driver of PGD leading to inferior short- and long-term survival in LTx (54) demonstrated that PCLS can be used to study lung IRI by employing a hybrid model, with an in vivo ischemic period followed by ex vivo reoxygenation (54)

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By studying these spaces, researchers can gain insight into how mental health concerns manifest themselves in unstructured, natural conversations 13