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These initial human studies typically enroll a small number (20-100) of healthy volunteers

References 1 AbeK.ItoyamaY.SobueG.TsujiS.AokiM.DoyuM.et al

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GSH

Xue Y, Qu Z, Fu J, Zhen J, Wang W, Cai Y, Wang W (2017) The protective effect of astaxanthin on learning and memory deficits and oxidative stress in a mouse model of repeated cerebral ischemia/reperfusion