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Utilizing Light Cycler 480, SYBR Premix Ex Taq TM II, and gene-specific primer pairs, quantitative real-time PCR was carried out using generated cDNAs as templates (Roche)

Sometimes we must focus on the liver as the first step, to start being livers of life again

We also thank the anonymous peer reviewers for their constructive and insightful feedback, which greatly improved the quality of this manuscript

Statistical analysis The sample size was calculated to detect a 20% increase in venous cord blood GSH concentration, targeting 639 mol/L in infants born to NAC-supplemented mothers, compared with the median of 555 mol/L in untreated preterm infants