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Hcy and Hcy thiolactone promote arteriosclerosis via increased oxidant stress, impaired endothelial function, and induction of thrombosis, increasing the cardiovascular disease risk in humans (43)

Concurrently, certain inhibitors have been identified as effective agents in the attenuation of ROS-mediated mitochondrial apoptosis

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However, in a controlled randomized study, NAC was found to be ineffective in preventing the deterioration in lung function and exacerbations in patients with COPD [76]

To generate HCT116-HiBiT-BRD4 knock-in cell lines in which the HiBiT tag was inserted into the N-terminus of BRD4, a CRISPR guide sequence targeting exon 2 of BRD4 was cloned into pSpCas9(BB)2A-Puro (PX459) v.2.0, which was a gift from F