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After binding his-tags to the nickel beads, the beads are washed, then eluted by raising the concentration of imidazole in the elution buffer

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Different histamine receptors are found in different parts of the body:[ref] H1 receptors : Found in smooth muscle, endothelial cells (lining the blood vessels), the central nervous system, and mast cells

doi: 10.1016/j.jhep.2012.03.024

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