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FIGURE 2 3 ECD ECD is defined as a series of maladaptive endothelial functional phenotypic changes associated with an increased risk of cardiovascular diseases (Figure 3) (Rajendran et al., 2013

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Mitochondrial staining and reactive oxygen species (ROS) detection For mitochondrial staining, after washing once with PBS, 5 mM MitoTracker (Thermo Fisher Scientific) and 200 mM Hoechst (Thermo Fisher Scientific) were added to the cells