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The L45 intervertebral disk was desiccated with diffuse disk bulging causing severe reduction of both L45 intervertebral foramina and severe spinal canal stenosis at the same level

These effects on hypothalamic catecholamine regulation contribute to restoration of proper circadian signaling needed for reproductive hormone surges and metabolic timing

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Maxisorp 96-well plates (Thermo Fisher Scientific) were coated with 2.5 g/ml recombinant metE in PBS and incubated overnight at room temperature