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As a result, immune and metabolic functions normally performed by beneficial bacteria are impaired, detrimental effects associated with the presence of opportunistic bacteria and pathogens are increased, and the host becomes more vulnerable to diseases and infections (47)

Further in-depth studies should be carried out to confirm the molecular mechanisms

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The First-Strand cDNA Synthesis SuperMix Kit (TransGen Biotech, Beijing, China) was used to synthesize the first-strand complementary DNA (cDNA), following the manufacturers instructions

[DOI] [PMC free article] [PubMed] [Google Scholar] Weibel KE, Mor JR, Fiechter A