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Microscopy Most high resolution fluorescence micrograph data were captured on Zeiss Axiovert Z1 microscope equipped with X-Light V2 Spinning Disk Confocal Unit (CrestOptics), 7-line LDI Laser Launch (89 North), Photometrics Prime 95B Scientific CMOS camera, using Metamorph 7.7 software

The reason for this mild profile is partly biological

Importantly, given that mild fasting results in energy stress and a decline in the levels of growth factors, leading to increased AMPK and GSK3 activity, it can be predicted that fasting would boost SIRT7 functions in normal tissues/organs, as has been reported previously in liver 20

Soaking could increase the protein solubility of yellow pea flours to some extent, possibly due to proteolytic enzyme breakdown of proteins

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