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Chromatographic separation was achieved on a Platinum EPS C18 100 A 3 u (length: 53 mm, internal diameter: 7 mm, maximum pressure: 5000 PSIG) analytical column (Alltech Associates, Inc., Deerfield, Ill.) at ambient temperature with a flow rate of the mobile phase maintained at 1 ml/min

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Positive charge patterning and hydrophobicity of membrane-active antimicrobial peptides as determinants of activity, toxicity, and pharmacokinetic stability

4 Inject the water slowly into the peptide vial along the inner wall, not directly onto the powder

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