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The membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody (Catalog # A02059-1) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT

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All constructs were verified by sequencing

This is because vitamin B12 is involved in the production of red blood cells, which helps the skin produce its pigmentation

GABA abnormalities in schizophrenia: a methodological review of in vivo studies