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We recommend splitting and plating cells on a consistent schedule and in a manner where the cells are never too sparse or too dense

Gastroenterology 116:678685 Strasberg S (1995), Acute calculus cholecystitis

For selectivity studies, recombinant GSTO1 (1 M), PDI (1 M), BSA (1 M) or GRP78 (1 M) in reaction buffer were incubated with C1-27 or DMSO for 30 min at 37 C, followed by incubation with CMFDA (500 nM), BODIPY- C1-27A (1 M) or DMSO for 30 min