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A mutation in CLCNKB results in hypokalemia as seen in autosomal recessive Bartter syndrome type 3 (BS3) 5355

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Macrophages and white blood cells flood the damaged area, and the resulting tissue fluid exudation leads to erythema with a radius of 3 to 5 cm on the skin surface

Therefore, the study of individual components is inappropriate in this case

A suggested strategy is to use 2D cultures for rapid initial screening to identify relevant effects, and 3D models can be helpful for further studies of efficacy or cytotoxicity before using animal models [166]