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For experiments requiring permeabilization of DC2 cells during analyzer measurements, respiration was measured in a low potassium, low calcium buffer (3.5 mM KCl, 120 mM NaCl, 0.4 mM KH 2 PO 4 , 1.2 mM Na 2 SO 4 , 2 mM MgCl 2 , 20 mM Na-N-Tris-(hydroxymethyl)-methyl-2-amino-ethanesulfonic acid (TES pH 7.4), 1 mM EGTA, 0.4% wt/volume fat-free bovine serum albumin (BSA))

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A UV-absorptive aromatic QLD tag was selected for efficient tagging of GSH through a rapid thiol-ene click reaction

Pharmaceutics 15:2666