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As part of this pathological process, hepatic lipid accumulation and immune cell infiltration trigger the sustained release of proinflammatory cytokines and chemokines, such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-), driving a systemic inflammatory response [26, 29]

The wild-type is able to advert immune system by having all these properties and this will delay the activation of macrophages and the downstream signaling pathways ( BacA has been found to impair MAP growth in vivo ( BacA is not over-attenuated to get cleared before inducing immune responses, and it is not under-attenuated to persist in tissue and maintain a chronic infection

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In one aspect, this invention provides methods for improving the efficacy of genetic vaccines by providing materials that facilitate targeting of a genetic vaccine to a particular tissue or cell type of interest