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Description

However, the most clinically relevant role of CYP2E1 is associated with ALC and APAP metabolism, especially at their higher doses

For example, if this marker is green fluorescent protein, cells with the highest expression levels can be identified by flow cytometry-based cell sorting

Each of the 430 compounds from a SelleckChem Kinase Inhibitor Library (subset of library #L1200) were combined at 100 M in wells of a polypropylene 96-well Tube Plates (Agilent) with TcPINK1 D337N at 0.5 mg/mL (10 M) and SYPRO Orange Protein Gel Stain 5000X (Thermo Fisher Scientific) at 6X in a 300 mM NaCl, 20 mM TrisHCl pH 8, 5 mM DTT, 5% DMSO buffer