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This reduction lifts the inhibition on autophagy and triggers autophagosome formation, as evidenced by increased levels of LC3-II/I and Beclin-1, alongside a decrease in p62

Cell viability and mitochondrial health Cell viability test using the xCELLigence real-time cell analyser To determine the cellular response to B-017 treatment in different cell lines exposed to stimulation of cell death signalling, the cellular impedance was measured in 96-well plates equipped with gold microelectrodes using the xCELLigence Real Time Cell Analyser Cardio system (Agilent Technologies, USA, OLS, Bremen, Germany) placed in an incubator at 37 C with 5% CO 2