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Since cell quantification was not possible due to the organization of WT differentiated cells into tight aggregates, to quantify the differences between Phf3 and Dido WT and KO cells upon neuronal differentiation we manually counted by fluorescence microscopy all TuJ1 positive cell aggregates (referred to as neuronal clumps) on the glass coverslips

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Neuron 68:557569 Kovoor A, Nappey V, Kieffer BL, Chavkin C (1997) Mu and delta opioid receptors are differentially desensitized by the coexpression of beta-adrenergic receptor kinase 2 and beta-arrestin 2 in xenopus oocytes

1 Introduction Inflammatory bowel disease (IBD) is a group of diseases characterized by chronic inflammation of the gastrointestinal (GI) tract, with periods of exacerbation and remission