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Finally, although the correlations between the metabolome and microbiome were established using robust statistical analyses, there was limited statistical power and there are also limitations to the statistical approaches used herein (e.g., log transformation to resolve non-normal distribution and/or heteroscedasticity 103 )

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The soluble fraction was loaded onto a nickel affinity column formed from nickel sepharose beads (GE Biosciences) pre-equilibrated with lysis buffer

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