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Cells were incubated for 10 min at room temperature and then 20 L of 10% CHAPS detergent (Sigma-Aldrich, C3023) was added, and cells incubated at room temperature for an additional 10 min to lyse cells

and which of the Cys was more likely to be modified

Further exploration of the specific mechanisms linking ferroptosis to disease progression will deepen our understanding of these significant diseases and provide new strategies for more precise prevention and treatment options for patients

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Analytical Verification and Review Identity, purity, and structure-relevant data are reviewed against the project objective so the resulting package supports practical decisions rather than only confirming reaction occurrence