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Therefore, heparin binding by apo-HPX at acidic pH in vitro likely represents an interaction between apo-HPX and a membrane-associated HSPG in vivo rather than an effect on heme binding to HPX because absorbance spectroscopy of ligand binding to hemopexin showed that heme competed effectively with heparin (80-fold molar excess) for HPX binding (Figure 8D)

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