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Briefly, RPL22Flag-expressing cells from shCtrl, shGDH1 and shGDH1 + -KG groups were treated with 100 g ml 1 CHX for 15 min before lysis and anti-Flag beads (Biomag, BMFA1000-2) were used to capture the RNARPL22 complex

We first examined the effect of sertraline on the cell proliferation of A549 cells and found that NKX21 overexpressing A549 cells were sensitised to sertraline in serine/glycine-depleted conditions (Fig