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This process was fundamental in order to buffer ROS flux and a consequent detrimental over increase of self-digestion (Desideri et al., 2014

After microscopic examination, oocytes at metaphase II stages (MII, with the polar body extrusion) were collected and cultured in 500 l of fertilization droplets as SOF medium supplemented with 5% fetal bovine serum, 2 mM sodium pyruvate, 10 g/ml heparin sodium and 1 mM caffeine in a CO 2 incubator (38.5C, 90%N 2 , 5%CO 2 and 5%O 2 ) (32)

Most inflammatory cytokines, like interleukin-6 and TNF-, are elevated in both women with HELLP syndrome [79] and their newborns [80]

Glutathione actively combats free radicals, thereby preventing cellular damage and aging

Female mice, aged 6 to 8 weeks, from these backgrounds were used in all experiments