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When pernicious anaemia is suspected, testing for anti-intrinsic factor antibodies (and sometimes anti-parietal cell antibodies) is recommended

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Embedded brains were stored at 80 C until use and sectioned on a Leica CM3050S cryostat, collecting 14 m sections at every 100 m on Superfrost Plus glass slides (Thermofisher Scientific, Waltham, MA)

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