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For intracellular cytokine staining, cells were incubated with Brefeldin A (1:1000, #00-4506-51, Invitrogen, USA), Monensin (1:1000, #00-4505-51, Invitrogen, USA), PMA (10 ng/mL, #P8139, Sigma, USA) and ionomycin (500 ng/mL, #FMS-FZ208, Fcmacs, Nanjing, China) at 37 C for 3.5 h

Given the multifactorial nature of PCOS, therapeutic approaches require personalized strategies rather than single-modality interventions

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In addition, understanding the mechanisms by which exercise interplays with psychological therapies and medications will offer important insights into how to optimize treatment plans for individuals with PTSD