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In addition, we carried out pilot studies to isolate hemeHPX-binding proteins (i.e., putative receptors and other biologically relevant HPX-interacting proteinsan interactome) using hemeHPX affinity chromatography of whole-cell extracts from three different human cell types: human promyelocytic HL-60 cells, human hepatoma HepG2 cells, and freshly isolated, plated human hepatocytes

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Myeloperoxidase (MPO) staining The immunohistochemistry of MPO was conducted using mouse anti-MPO antibodies (Servicebio, Wuhan, GB12224) and HRP-labeled goat anti-mouse antibodies (Servicebio, Wuhan, G23301) along with DAB chromogenic agent kit for histochemistry (Servicebio, Wuhan, GB12224)