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1) forming branched chain keto acids and pyruvate from the branch chain amino acids and alanine, respectively

However, AARS is an exception, which relies on lactate-AMP as an intermediate to directly transfer lactyl group to the lysine residues of target proteins with the assistance of ATP [25, 26] As the most well-studied writer, p300-catalyzed lactylation mainly promotes gene transcription [28,29,30,31,32,33,34,35,36,37,38,39] and maintains normal protein structure and functions [40,41,42,43,44,45]

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