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Subsequently, fungal cells were stained with Calcofluor White (Sigma-Aldrich) at a final concentration of 1 g/ml and Sytox Orange (ThermoFisher Scientific, Waltham, MA, United States) at a final concentration of 1 M for 10 min in the dark

while the internal changes are the cell ageing acceleration, regeneration capacity weakness and pigmentation

After incubation for 15 min at room temperature, both solutions were combined and added dropwise to the cells combined

ST: Diet and mental health research in the Thuret lab is funded by grants awarded by the Medical Research Council UK (MR/N030087/1 and MR/S00484X/1, the European Unions H2020 Joint Programming Initiative A Healthy Diet for a Healthy Life and the Network of Centres of Excellence in Neurodegeneration (COEN)

This restoration not only improves hyperpigmentation directly but also prevents future pigmentation problems by maintaining skin health and reducing inflammatory triggers that activate melanocytes