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on 24 patients with generalized vitiligo and 20 healthy controls found statistically significant higher levels of superoxide dismutase activity in erythrocytes, serum MDA, and nitric oxide (NO) and statistically significant lower levels of glutathione and glutathione peroxidase in the patients of vitiligo compared to the controls.[] Secondary to the oxidative stress, the polyunsaturated fatty acids undergo peroxidation resulting in formation of free radicals

Both groups were housed in a hypoxic incubator (oxygen concentration: 16%, simulating 20002200 m altitude) [31]

The three IFN products were tested based on known immunomodulatory and anti-viral effects of type 1 IFN

Formazan crystals were solubilized in 100 L of DMSO by incubating in shaking condition at room temperature for 5 min