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It has been shown that modulation of intracellular Ca 2+ levels in single-cell cultured astrocytes by microinjection (Araque et al., 1999) of the Ca 2+ chelator BAPTA [1,2-bis(2-amino phenoxy) ethane- N, N, N9, N9 -tetraacetic acid] or by photolysis of the UV sensitive Ca 2+ -cage (Araque et al., 1999), or with o-Nitrophenyl-Ethylene glycol tetraacetic acid (NP-EGTA), influences the spontaneous neurotransmitter release from the nerve terminals (Araque et al., 1999)

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10.1124/mol.63.2.351 16 ChengY.ChenY.ZhouC.ShenL.TuF.XuJ.et al (2020)

Although there is no reason to believe that a neutral antagonist would directly affect MC3R signaling, the possibility remains that such an antagonist may lead to cross-sensitization or reduced internalization of the dimer, in effect amplifying MC3R signaling further through increased surface expression or sensitization