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Chromatographic separation was achieved using a Waters Acquity UPLC BEH C18 column (1.7 m, 2.1 50 mm) with a VanGuard pre-column of the same chemistry (Waters Co, Milford, MA), and retention times for speciogynine and paynantheine were 1.0 and 2.1 min, respectively

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Gut microbes produce or metabolize a spectrum of bioactive compounds, including SCFAs, bile acids, and tryptophan derivatives, which collectively influence energy homeostasis, insulin sensitivity, and sex hormone balance

We take the K m of GST for GSH to be 5200 M, midway between the values 4500[21] and 5600[22], K m = 15 M for NAPQI, and V max = 72,000 M/hr

Similarly, a recent meta-analysis of 15 cohort studies with over 86,000 PD cases and nearly 30 million subjects, found that prediabetic patients have a 4% increased risk of PD compared to individuals with normal blood glucose level [20]