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Healthy NP tissue generally contains two cell types, depending on age and species: notochordal cells that play an important role in IVD development, growth, and homeostasis, and the nucleopulpocytes that maintain the IVD post development (Clouet et al., 2019)

Controlled addition of ROS, typically 1050 M H 2 O 2 , to in vitro oocyte cultures is a widely used method for modeling oxidative stress [83]

Most water-soluble vitamins (including most B vitamins and vitamin C) also are absorbed by simple diffusion

Notably, cysteine is also essential in animals with mutations in either cystathionine -lyase (CSE, also known as CTH and CGL) or cystathionine -synthase (CBS), enzymes of the trans -sulfuration pathway 12,13 (Fig

This rate of hydrolysis increases with pH and is particularly important when dealing with dilute protein solutions