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Drugs such as capecitabine, methotrexate, and various tyrosine kinase inhibitors require intact renal or hepatic pathways for clearance

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Both drugs carry a black box warning for thyroid C-cell tumors, although this has primarily been observed in animal studies

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Cells were maintained in Dulbeccos Minimal Eagle Medium (DMEM) with 10% fetal bovine serum (FBS), Blasticidin, and Penicillin/Streptomycin and then transferred to 1.5 ml freezing vials and stored under liquid nitrogen in growth media containing 5% DMSO