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A complementary axis involves transcriptional reinforcement of antioxidant programs, including NFE2 like BZIP transcription factor 2 (NFE2L2, also known as NRF2) signaling, which increases GPXs and further suppresses ROS-driven inflammasome activation 58

One of the more commonly used methods to induce disc degeneration in recent studies has been the needle puncture injury method in both in vivo and ex vivo small and large animal models, where the IVD is punctured using needles of various gauges (Korecki et al., 2008

In animals, the arteries are the pathways of these transfers to the cells

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No approved indication