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H.KimC.-B.NohS

Ubiquitin modification was detected by western blotting using anti-OTUD5 antibody or CBB stain (BIO CRAFT)

Pat the area dry with a clean towelnever rub vigorously

Hepatoprotective effects of green tea and its polyphenols: preclinical observations

Although a limitation is that halogenated compounds containing chloride ions (e.g., Tris-HCl, NaCl) cannot be used in the lysis or binding buffers, the system offers the advantage of one-step purification (~1 h) with high specificity, as has been reported in several applications involving recombinant protein expression and purification using this system [43,44,165,166]