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Briefly, cells were seeded into 24-well plates (pre-coated with poly-l-Lysine) in RPMI 1640 complete media at 5 10 5 cells/well and cells were cultured overnight (37C, 5% CO 2 )

Importantly, we found that CHAC1 expression is significantly upregulated in skeletal muscles under various wasting conditions, including cancer induced cachexia, chemo-agent cisplatin induced cachexia, and fasting induced muscle atrophy

Next, both protein and prepared standard samples were added sequentially to the enzyme standard plate following the instructions provided in the kit

In contrast, T-GSH content was positively correlated with the cysteine content (r=0.87), and the activity of GPX (r=0.64) and MDHAR (r=0.66)

Chalmers A