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Final Report

The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2

In a frequently cited 1999 rat wound study in the Journal of Investigative Dermatology, topical Thymosin Beta-4 was associated with a 42% increase in reepithelialization at four days and as much as 61% at seven days compared with saline controls, alongside increased angiogenesis

Their seemingly miraculous properties have been praised by tech CEOs, podcaster Joe Rogan, and celebrities such as Gwyneth Paltrow, Jennifer Aniston, and Kim Kardashian, who says her daily regimen "changed my life." But many doctors are alarmed by a craze that has thousands of people injecting themselves with substances of dubious origin and whose long-term effects are unknown