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The resulting sections were mildly fixed with 2% paraformaldehyde (VWR Life Science) for 10 min, permeabilized by incubation in 0.05% Triton X-100 for 10 min and blocked with 5% BSA for 1 h at room temperature

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On days 5 and 12 of the BM assay short-term memory (STM) and long-term memory (LTM), respectively, were tested, evaluating how well the mice remembered the location of the target hole

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The in-depth analyses of the results revealed that the proteins enolase, neuropolypeptide h3, ATP synthase, and fructose bis-aldolase (FBA) were identified as oxidized in brain from subjects at each stage of AD, late-stage AD, EAD, and MCI, consistent with the concept that their oxidation is an early event in and may contribute to progression of AD