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Nonetheless, certain individuals might encounter mild adverse effects including nausea, bloating, and certain dermal issues like itching and increased perspiration

After tandem purification using GST and strep affinity tags, we obtained the protein sample for cryo-EM data collection (Fig

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After centrifugation, the supernatant solution was collected and purified using semi-preparative HPLC, C4 column and gradient of 2060% B to give the desired product in ~45% yield (Supplementary Fig