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The absorbance was measured at 450 nm with a microplate reader

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Importantly, oleic acid exhibits model- and exposure-dependent bidirectionality: intravenous oleic acid is widely used as a lipotoxic trigger in experimental acute organ injury and has been reported to induce lung and kidney injury, whereas certain interventions (e.g., AT1R blockade) may partially mitigate oleic acid-induced tissue damage in specific settings (Talebi et al

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