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cDNA was then used as template for RT-qPCR with a QuantStudio 3 Real-Time PCR system (Applied Biosystems) using PowerUP SYBR Green Master Mix (Applied Biosystems) and 500 nM of target gene specific forward and reverse primers, using the recommended fast cycling mode

Every lot is also screened for more than 450 banned substances with surveillance testing for environmental contaminants like heavy metals, microbiological agents or pesticides

Real-time qRT-PCR Real-Time qRT-PCR was conducted as described previously 46

To support our post-mortem findings, we used in vitro models (LUHMES neurons and mouse cortical neurons (PCNs)) and an -syn overexpression C

With more severe injury, synapse disruption, axonal injury, excitotoxicity, and cell death also become major effectors