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doi: 10.3390/IJERPH20010235 206

The OxICAT method was performed according to the protocol of Lindemann and Leichert 25, 26 with the modification that cells were disrupted using a ribolyzer

Considering the well-established role of metabolic reprogramming in macrophage activation phenotypes, we evaluated the impact of NOS2 deficiency on MPLA-induced metabolic shifts by Seahorse extracellular flux analysis