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For targeted gene studies, information was recorded on whether the region was hypomethylated, hypermethylated, or did not show differences in the different comparisons

The day after, dead cells were removed through medium washes prior being treated with different concentrations of CuCl 2 to assess resistance to copper by Live/Dead or apoptotic assays (see below)

Although not much is known about these enzymes in cancer, the CHAC2 isoform has already been classified as a tumour suppressor gene in gastric and colorectal cancer, on the basis of its downregulation being associated with more aggressive cancer variants and its activation-inducing apoptosis in cancer cells