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In each permutation, a PLS-DA model was built between the data (X) and the permuted class labels (Y) using the optimal number of components determined by cross validation for the model based on the original class assignment

(2019) 29:116681.e6

Specifically, CAR T cells in the day 28 BM of LTR had significantly higher expression of mTOR, pS6, pAKT (mTOR and pAKT pathways) and the metabolic sensor AMPK, as well as significant increases in the fermentative glycolysis enzyme LDHA, the endogenous fatty acid metabolism enzymes SCD1, LAL, and CD45RA, suggesting an effector immunometabolic phenotype